RealSeq®-biofluids
RealSeq®-biofluids kits for 12 libraries: catalog number 600-00012
RealSeq®-biofluids kits for 24 libraries: catalog number 600-00024
RealSeq®-biofluids kits for 48 libraries: catalog number 600-00048
Order Now (UK customers, please order through www.bioscience.co.uk)
RealSeq®-AC kit contents
Box 1
| Tube | Reagent | Storage |
|---|---|---|
| RB | RNA buffer | -20°C |
| A | RealSeq® Adapter | -20°C |
| HY | Hy4 Removal Probe | -20°C |
| RI | RNAse Inhibitor | -20°C |
| LB | Ligation Buffer | -20°C |
| L | Ligase | -20°C |
| BA | Blocking Agent | -20°C |
| BE | Blocking Enzyme | -20°C |
| RSE | RealSeq® Enzyme | -20°C |
| RSB | RealSeq® Buffer | -20°C |
| DRA | Dimer Removal Agent | -20°C |
| RTP | RT Primer | -20°C |
| dNRP | dNTPs | -20°C |
| RTB | RT Buffer | -20°C |
| RTE | RT Enzyme | -20°C |
| PB | PCR Buffer | -20°C |
| PP | PCR Polymerase | -20°C |
| W | RNase-free Water | -20°C |
| AD | Adapter Dilution Buffer | |
| + | miRNA Control | -20°C |
Box 2: Index Primers
| Tube | Reagent | Storage |
|---|---|---|
| FP | Forward Primer | -20°C |
| RP 1 to 12* | Reverse Primer, Index 1-12 * | -20°C |
Beads
| Tube | Reagent | Storage |
|---|---|---|
| B | RealSeq®-AC Beads | 4°C |
| SPRI | SPRI Select Reagent | 4°-25°C |
User-supplied Reagents
- 70% Ethanol (molecular biology grade - Freshly prepared)
- Bioanalyzer DNA 1000 kit (Agilent #5067-1504) or Tape Station D1000 DNA kit (Agilent #5067-5582 & #5037-5583)
- Qubit® dsDNA HS Assay Kit, 100 assays (Thermofisher Scientific #Q32851)
Equipment and consumables needed
- Pipettes and nuclease-free filtered pipette tips for volumes 2 to 100 µl.
- RNAse-free microfuge tubes (1.5 ml)
- PCR tubes or PCR strip tubes
- Ice bucket/freezer plate for reagents and enzymes
- Bioanalyzer (Agilent) or Tape Station (Agilent)
- Qubit Fluorometer (ThermoFisher Scientific)
- Thermal Cycler
SDS
Legal and Patent Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by SomaGenics, Inc. No right under any other patent claims to methods, apparatus or reagents is conveyed expressly, by implication, or by estoppel. This product is intended for Research Use Only and may not be transferred to third parties, resold, modified for resale or used to manufacture commercial products without prior written approval of SomaGenics, Inc. For more information about commercial rights, please contact SomaGenics Business Development at .