RealSeqKit

miR-ID® Kits

Currently available miR-ID® kits:   pdfmiR-IDassays-Oct17.pdf

New miR-ID® assays are continually being added to our catalog. If you do not see your favorite miRNA on our list or are interestd in detecting novel isoforms, please inquire; it may be available shortly or by special order. Send all inquiries regarding products or ordering to: .

Order Now   (UK customers, please order through www.bioscience.co.uk)

Price for each catalog kit: $170 (Please inquire about pricing for custom kits)

miR-ID® kit contents

  1. Circularization Reaction Mix
  2. RT primer/s (for 100 reactions)
  3. RT Reaction Mix
  4. RT dilution Buffer
  5. Nuclease-free Water
  6. PCR primers (for 300 reactions)

User-supplied Reagents

  • CircLigase II (Epicentre, cat# CL9021K)
  • RNAseOUT (Thermo Fisher Scientific, cat# 10777-019)
  • Superscript IV Reverse Transcriptase (Thermo Fisher Scientific, cat# 18090010)
  • 2X iQ SYBR Green Supermix (BioRad, cat# 170-8880)

Equipment and consumables needed

  • Pipettes and nuclease-free filtered pipette tips for volumes 2 to 100 µl.
  • RNAse-free microfuge tubes (1.5 ml)
  • PCR tubes or PCR strip tubes.
  • Micro-Amp Fast Optical 96-Well Reaction Plate, for example Thermo Fisher Scientific cat# 4346906
  • Micro-Amp Clear Adhesive Film, for example Thermo Fisher Scientific cat# 4360954
  • Ice bucket/freezer plate for reagents and enzymes
  • Centrifuge for 96-well plates
  • Thermal Cycler
  • Real-time qPCR instrument, for example 7500-Real Time PCR System (Thermo Fisher Scientific)
 

Legal and Patent Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by SomaGenics, Inc. No right under any other patent claims to methods, apparatus or reagents is conveyed expressly, by implication, or by estoppel. This product is intended for Research Use Only and may not be transferred to third parties, resold, modified for resale or used to manufacture commercial products without prior written approval of SomaGenics, Inc. For more information about commercial rights, please contact SomaGenics Business Development at .